Fig. 10

BMP2 upregulation in LSCC results from increased transactivation by RUNX1. A CNV analysis of 12 prognostic VMRGs. B Venn diagram of predicted transcription factors. C Heatmap of correlation analysis. D, E QRT-PCR and western blotting shows the overexpression of RUNX1 in LSCC cells. F Diagram of RUNX1 binding sites. G qRT-PCR of the expression of RUNX1 and BMP2. I Western blot analysis of BMP2. J Immunofluorescence assay, RUNX1 (red fluorescence); BMP2 (green fluorescence). K Upon shearing by sonication, chromatin fragments ranged from 100 to 500 bp in size. L Western blotting was used to detect the RUNX1 in the ChIP assay. M DNA-binding sites electrophoresed on agarose gels. N Dual-luciferase reporter assays were used to analyze luciferase activity. O–P The levels of expression of BMP2 and RUNX1 were correlated according to IHC staining. Q Multivariate analysis of RUNX1 **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA and two-way ANOVA