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Table 1 Chemicals and reagents used in the study

From: Interleukin-4 protects retinal ganglion cells and promotes axon regeneration

Chemicals

Source

Identifier

Dosage

Staurosporine

Abmole (Beijing, China)

Cat# M2066

 

N-methyl-D-aspartic Acid (NMDA)

Abmole

Cat# M2884

 

4’,6-diamidino-2-phenylindole (DAPI)

Solarbio (Beijing, China)

Cat# C0065

 

Antifading mounting medium

Solarbio

Cat# S2100

 

Cholera Toxin Subunit B (CTB) conjugated Alexa Fluor 488

Thermo Fisher Scientific (MA, USA)

Cat# C34775

 

recombinant murine IL-4 protein

PeproTech (Rocky Hill, NJ, USA)

Cat# 214 − 14

 

Reagents for cell histology

Source

Identifier

Dosage

mouse anti-βIII-Tubulin

Beyotime (Shanghai, China)

Cat# AT809

1:100

rabbit anti-NeuN

Abcam (Cambridge, UK)

Cat# ab177487

1:400

DyLight 488, anti-mouse IgG

Abbkine (Shanghai, China)

Cat# A23210

1:400

Cy3, anti-rabbit IgG

Abbkine

Cat# A22220

1:400

Reagents for Western blot

Source

Identifier

Dosage

mouse anti-β-III Tubulin

Beyotime

Cat# AT809

1:2000

rabbit anti-NeuN

Abcam

Cat# ab177487

1:5000

mouse anti-β-actin

Signalway (Pearland, TX, USA)

Cat# 21,800

1:8000

anti-rabbit IgG, HRP conjugated

Signalway

Cat# L3012

1:8000

anti-mouse IgG, HRP conjugated

Signalway

Cat# L3032

1:8000

Reagents for tissue histology

Source

Identifier

Dosage

rabbit anti-RBPMS

OmnimAbs (California, USA)

Cat# OM165217

1:200

rabbit anti-GAP43

Beyotime

Cat# AF0153

1:200

mouse anti-β-III Tubulin

Beyotime

Cat# AT809

1:100

rabbit anti-IL4R

Affinity Biosciences

Cat# DF8567

1:200

DyLight 488, anti-rabbit IgG

Abbkine

Cat# A23220

1:400

DyLight 488, anti-mouse IgG

Abbkine

Cat# A23210

1:400

Cy3, anti-rabbit IgG

Abbkine

Cat# A22220

1:400