Fig. 5
From: Interleukin-4 protects retinal ganglion cells and promotes axon regeneration
Recombinant IL-4 protein reduced RGC cell death caused by NMDA injury. A: Immunofluorescence staining of retinal ganglion cells in the negative control group, vehicle group and rIL-4 group on day 7 and day 14 showed that the density of RGCs in the rIL-4 group was higher than that in the vehicle group at the same treatment time. B: Schematic diagram of the RGC counting method. A rectangle (red) indicates a high-power field of 0.38 mm×0.285 mm. The number of RGCs in each high-power field was counted, and the mean RGC number in a high-power field was calculated. The proportion of the mean RGC number of the vehicle group or rIL-4 group relative to the mean RGC number of the negative control group was calculated as the relative RGC survival rate. C: The relative RGC survival rate of each group. The relative RGC survival rate of the IL-4 group was greater than that of the vehicle group with the same treatment time. n = 9 eyes per group, *** indicates p < 0.005, **** indicates p < 0.001