Fig. 8
From: MiR-574-5p activates human TLR8 to promote autoimmune signaling and lupus
In vivo silencing of miR-574-5p in the lupus-prone mice causes significant changes in Stat1 signaling and activation of B lymphocytes. In vivo silencing of miR-574-5p was achieved by treatment with lentiviruses carrying shRNA against miR-574-5p as described. NS, not significant; * P < 0.05. (a) Silencing of miR-574-5p significantly attenuated Stat1 signaling (n = 4). (b) Silencing of miR-574-5p significantly attenuated lupus-associated activation of B cells (n = 4). B cell activation was analyzed by flow cytometry gated on B220+Gl7+ or B220+Cd69+. (c) Silencing of miR-574-5p slightly but not significantly attenuated lupus-associated activation of Tfh cells (Cxcr5hiPd1hi Cd4+, n = 4). Cd4+ splenocytes were isolated by FACS. Tfh cell activation was analyzed by flow cytometry gated on Cxcr5hiPd1hi. (d) Typical results showing reduced formation of splenic Spt-GC following in vivo silencing of miR-574-5p (n = 2–3). Spleen tissues were dissected and stained with antibodies against IgD and peanut agglutinin (Pna) as described [32]. Brown staining indicated germinal center B cells (IgD−Pna+) while blue staining indicated follicle B cells (IgD+Pna−). Scale bar, 100 μm