Fig. 4
From: MiR-574-5p activates human TLR8 to promote autoimmune signaling and lupus
miR-574-5p-mediated immune and inflammatory responses is mTlr7- and MyD88-dependent in mouse cells. NS, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001. (a) miR-574-5p or miR-574-5p + uridine exposure significantly induced Tnfα secretion in MyD88+/+ but not MyD88−/− RAW264.7 macrophage cells. About 2 × 105MyD88+/+ or MyD88−/− RAW264.7 cells were treated with Dotap only, 10 µg/ml Dotap-PS-miR-574-5p or 10 µg/ml Dotap-PS-miR-574-5p + 4 mM uridine respectively for 24 h (n = 4). (b) miR-574-5p overexpression significantly increased serum Tnfα and Il6 levels in the B6.WT mice but not the B6.Tlr7−/− mice (n = 4–5). (c) miR-574-5p exposure significantly increased Stat1, pStat1Y701 and Trim21/Ro52 in splenocytes prepared from the B6.WT mice but not the B6.Tlr7−/− mice (n = 4–5)