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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Csn5 inhibits autophagy by regulating the ubiquitination of Atg6 and Tor to mediate the pathogenicity of Magnaporthe oryzae

Fig. 5

MoCsn5 negatively regulates autophagy. A Subcellular localization of GFP-MoAtg8 in 70-15 and ΔMocsn5 under nutrient and starvation conditions. Bar: 10 μm. Mycelia were stained with CMAC to label the vacuoles. B Autophagic flux analysis of GFP-MoAtg8 in 70-15 and ΔMocsn5. Total GFP-MoAtg8 and free GFP were detected by western blot. The gray values of the protein bands were analyzed with ImageJ software. The degradation rate was expressed as [GFP/(GFP + GFP-MoAtg8)]. The protein content of actin was used as a control. C Analysis of MoAtg8/MoAtg8-PE turnover in 70-15 and ΔMocsn5. The ratio of MoAtg8-PE to actin was calculated to assess the rate of MoAtg8 lipidation to yield MoAtg8-PE

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Cell Communication and Signaling

ISSN: 1478-811X