Fig. 3
ΔMocsn5 is defective in the maintenance of external stresses. A and B Colony morphology and relative growth rate of 70-15, ΔMocsn5 and Mocsn5-C on CM supplemented with 2 mM hydrogen peroxide (H2O2) and 100 μM menadione (VK3). ***P < 0.001. C and D Colony morphology and relative growth rate of 70-15, ΔMocsn5 and Mocsn5-C on CM supplemented with 2 μM amphotericin B (AmB) and 1.5 μM myriocin. ***P < 0.001. E and F Colony morphology and relative growth rate of 70-15, ΔMocsn5 and Mocsn5-C on CM supplemented with 0.4 M KCl, 0.6 M NaCl, and 0.8 M sorbitol. Pictures were taken at 9 days. The data were analyzed with GraphPad Prism 8.0 software. ***P < 0.001, **P < 0.01. (G) Phosphorylation level of MoYpk1 in 70-15 and ΔMocsn5. The strains were cultured in CM supplemented with 0.6 M NaCl before analysis. H Phosphorylation level of MoOsm1 in 70-15 and ΔMocsn5 strains. The strains were cultured in CM supplemented with 0.6 M NaCl before analysis. I Phosphorylation level of MoOsm1 in 70-15 and ΔMocsn5 strains. The strains were cultured in CM or treated with 2 μM amphotericin B before analysis