Fig. 2
MoCsn5 is involved in conidial germination, appressorium formation, and pathogenicity in M. oryzae. A Disease spots of detached barley leaves inoculated with mycelial plugs from the 70-15, ΔMocsn5 and Mocsn5-C strains. Leaves were cultured at 25 ℃ for 4 days after inoculation. B Mycelial plugs from the 70-15, ΔMocsn5 and Mocsn5-C strains were inoculated on detached rice leaves. C Disease symptoms on wounded leaves of rice inoculated with mycelial plugs of the 70-15, ΔMocsn5 mutant and Mocsn5-C strains. D Disease symptoms on wounded leaves of barley inoculated with mycelial plugs of 70-15, ΔMocsn5 mutant and Mocsn5-C strains. E Disease spots of detached barley leaves inoculated with conidial suspensions of the 70-15, ΔMocsn5 and Mocsn5-C strains. Leaves were cultured at 25 ℃ for 4 days after inoculation. F Conidial morphology of 70-15, ΔMocsn5 and Mocsn5-C. Bar: 10 μm. G Germ tube germination assays and appressorium formation assays on hydrophobic surfaces at 4 hpi. Bar: 10 μm. H Germ tube germination assays and appressorium formation assays on hydrophobic surfaces at 24 hpi. Bar: 10 μm. I Statistical analysis of germ tube germination rates of 70-15, ΔMocsn5 and Mocsn5-C when conidia were dropped onto hydrophobic surfaces at 4 hpi and 24 hpi. The data were analyzed with GraphPad Prism 8.0. ***P < 0.001. J Statistical analysis of the appressorium formation rates of 70-15, ΔMocsn5 and Mocsn5-C when conidia were dropped onto hydrophobic surfaces at 4 hpi and 24 hpi. The data were analyzed using GraphPad Prism 8.0. ***P < 0.001. K Phosphorylation analysis of Pmk1 in 70-15 and ΔMocsn5. Pmk1 phosphorylation and Pmk1 levels were detected with phospho-Pmk1 and Pmk1 antibodies, respectively. The protein actin was used as a loading control. L The conidial suspension (5 × 104 ml−1) was dripped onto isolated leaves and incubated in a humid chamber at 25 °C. After 3 days, the leaves were decolorized with methanol and observed under an optical microscope. Bar: 10 μm. M The infection rate was quantified and statistically analyzed using GraphPad Prism 8.0 software