Fig. 5
From: EP300-ZNF384 transactivates IL3RA to promote the progression of B-cell acute lymphoblastic leukemia
EP300-ZNF384 elevates the expression of CD123 in B precursor cells. (A) CD19-positive cells were enriched with magnetic beads from peripheral blood mononuclear cells of healthy volunteers. Empty vector, EP300-ZNF384-, and ZNF384-expressing vectors were transfected into CD19-positive cells by lentivirus-mediated gene transfer. After 48 h, cells were collected for reverse-transcription quantitative PCR assay to assess the transcription of IL3RA. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance. (B) Representative flow cytometry dot plots illustrating CD123 fractions in CD19 positive B precursor cells in response to EP300-ZNF384 or ZNF384 expression