Fig. 2

Increased expression of IL3RA by EP300-ZNF384 promotes B-cell acute lymphoblastic leukemia (B-ALL) cell proliferation with or without IL-3. (A) NALM-6 cells overexpressing EP300-ZNF384, ZNF384, and the empty vector were subjected to a colony forming cell (CFC) assay in the presence or absence of IL-3 (10 ng/mL). Scale bar: 50 μm. Data derived from A was used for quantitative analysis of colony size (B) and colony number (C). *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance. (D) The NALM-6 cells were co-transfected with the shRNA vector (shIL3RA or empty vector) and overexpression vector (EP300-ZNF384 or empty vector) via lentivirus-mediated gene transfer. Hygromycin B (400 µg/mL) and puromycin (2 µg/mL) double selected cells were subjected to CFC assay. Scale bar: 100 μm. (E) Data derived from D were used for quantitative analysis of colony size (diameter) and number. *P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance