Fig. 1
From: Kinesin KIFC3 is essential for microtubule stability and cytokinesis in oocyte meiosis
KIFC3 is essential for mouse oocyte meiotic maturation (A) Oocytes were immunolabeled with anti-α-tubulin (green) and anti-KIFC3 antibody (red), and Hoechst 33,342 was used to label DNA (blue). Bar = 20 μm. (B) Co-localization of KifC3 with Crest at MI and MII stages. Oocytes cultured to 8 h (MI) and 12 h (MII) were stained for KIFC3 (green), Crest (red), and DNA (blue). Bar = 10 μm. (C) Immunoblotting assays for the expression level of KIFC3 at different stages (GV, GVBD, MI, and MII) during mouse oocyte maturation. (D) The typical picture of control oocytes and KIFC3-antibody injection oocytes. Bar = 50 μm. (E) Quantitative analysis of GVBD rate and PB1 extrusion rate in control and KIFC3-antibody injection oocytes. Graph shows means ± sem of results obtained in 3 independent experiments. *P < 0.05