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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: The protein composition of exosomes released by prostate cancer cells is distinctly regulated by androgen receptor-antagonists and -agonist to stimulate growth of target cells

Fig. 2

Androgen treatment increases CD9 protein level and induces CD9 secretion. A Fluorescent intensity measurement with the LSM. Nucleus (blue), CD9 (green), TIMP2 (red). Scale bars indicate 5 μm. B The intracellular protein level was defined as the normalized total cell fluorescence set as relative to DMSO. The mean ± SEM values were calculated from the technical replicates (n = 15). A two-tailed unpaired Student’s t-test was performed for statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001). C The secretion of CD9 from the same cells using signals in (A) detected outside of cells was measured using the LSM. Scale bars indicate 5 μm. D The secreted intensity of CD9 was determined. The mean ± SEM values were calculated from the technical replicates (n = 15). A two-tailed unpaired Student’s t-test was performed for statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001). CD9 (cluster of differentiation 9), LSM (laser scanning microscope)

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Cell Communication and Signaling

ISSN: 1478-811X