Fig. 6

Mitochondrial OXPHOS facilitates ferroptotic cell death in C. tropicalis-stimulated MDSCs. WT MDSCs were stimulated with C. tropicalis (MOI = 1) in the presence or absence of FCCP (5 µM) or Dimethyl 2-oxoglutarate (α-KG) (8 mM) for 24 h. (A and B) The percentage of cell death was determined using 7AAD. (C) The relative cell viability was detected by CCK-8 kit. (D and E) The Lipid ROS production was measured by BODIPY 581/591 C11 staining followed by flow cytometry. (F) The protein level of 4HNE was determined by western blot. (G) The relative MDA level was detected by MDA Assay Kit. Data with error bars are expressed as mean ± SEM, n = 3. Each panel shows at least three independent biological replicates from a typical experiment. Statistical analysis was determined by the unpaired Student’s t-test. ns (not significant), P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001