Fig. 5

Inhibition of SLC7A11 promotes OXPHOS of MDSCs upon C. tropicalis stimulation. (A-H, K-N) WT MDSCs were stimulated with C. tropicalis (MOI = 1) in the presence or absence of Sulfasalazine (1mM) or Erastin (5 µM) for 24 h. Mitochondrial function and mass, mitochondrial membrane potential were assessed by a flow cytometry assay (A-D). Oxygen consumption rate (OCR) of MDSCs was detected by Seahorse Cell Mito Stress Test (E and G). Basal respiration, ATP production and spare respiratory capacity (SRC) were calculated based on Seahorse Cell Mito Stress Test (F and H). The indicated proteins were measured by Western blot analysis (K and L). The levels of intracellular glutamate and α-KG were detected by assay kit (M and N). (I and J) qPCR analysis of the indicated genes in WT MDSCs treated with C. tropicalis (MOI = 1) in combination with or without Sulfasalazine (1mM), Erastin (5 µM) for 6 h. Data with error bars are expressed as mean ± SEM, n = 3. Each panel shows at least three independent biological replicates from a typical experiment. Statistical analysis was determined by two-way ANOVA with Bonferroni’s multiple comparisons test for post-hoc test and the unpaired Student’s t-test. ns (not significant), P > 0.05; *P < 0.05, **P < 0.01, ***P < 0.001