Fig. 7

High VDR expression correlates with increased M2 macrophages infiltration in tumor tissues. (A-B) Representative composite image of a TMA core with Opal six-color multiplex staining. Pseudocolor illustrating CD68 (cyan), VDR (green), Panck (white), CCL20 (yellow), CD86 (red), CD163 (orange), and DAPI (blue) staining. Scale bars = 100 μm. (C-F) Comparison between different VDR expression groups of panCK + cell density, CCL20 + cell density, M2/M1 macrophage within 200 μm of the VDR positive cells in tumor and stroma. (G-H) Comparison between different CCL20 expression groups of M2/M1 macrophage within 200 μm of CCL20 positive cells in tumor and stroma. (I) Subcutaneous graft tumors interfering with CCL20 expression in the Pan02 cell line overexpressing VDR. (J) Proliferation curve of tumor volume. (K) Staining and flow cytometric analysis of M2/M1 infiltration across different CCL20 expression groups. (L) Subcutaneous tumors after intervention of CCL20 expression on Pan02 with knockdown of VDR expression. (M) Subcutaneous graft tumors after treatment with local injections of IgG and CCL20 antibodies administered in the Plvx control and VDR overexpression groups, respectively. (N) Proliferation curve of tumor volume. Values presented in the graphs represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001