Fig. 6

High VDR expression correlates with increased M2 macrophages infiltration in vivo. (A) Protein expression levels of VDR in normal pancreatic tissues, Pan02 cell line, and the overexpressed VDR Pan02 cell line. (B) Subcutaneous graft tumors derived from the Plvx group or the VDR group. (C) The volumes of subcutaneous graft tumors were assessed twice a week. (D-G) Staining and flow cytometric analysis of macrophages, T cells, DCs and NKs infiltration across different VDR expression groups. (H) Immunofluorescence co-staining was performed to detect the M2 macrophages and vascular endothelial. Values presented in the graphs represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001