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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: VDR promotes pancreatic cancer progression in vivo by activating CCL20-mediated M2 polarization of tumor associated macrophage

Fig. 5

Blocking CCL20 rescues VDR-mediated macrophages polarization and infiltration. (A-B) Western blot and immunofluorescence results of CD163 following stimulation of macrophages with CCL20. (C) Representative images showing the influence of CCL20 on M2 macrophages migration assays. (D-G) ELISA assays measuring the secretion of VEGF, PDGF-AA, IL-10, and TGF-β by M2 macrophages. (H-K) CD163 was tested using qPCR, immunofluorescence, and western blot after co-culturing with condiction medium pretreated with IgG or anti-CCL20 antibody. Scale bars = 200 μm. (L) Representative images of M2 macrophages migration assays induced by condition medium. Scale bars = 200 μm. Values presented in the graphs represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X