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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: VDR promotes pancreatic cancer progression in vivo by activating CCL20-mediated M2 polarization of tumor associated macrophage

Fig. 4

VDR promotes the transcription and release of CCL20. (A) Heat map displayed the correlation between VDR and macrophage M2 polarization and recruitment gene set in TCGA PAAD data set. (B) Gene expression validation by qPCR. (C) The expression of CCL20 between normal and PAAD tissues. (D-E) Kaplan-Meier curves for CCL20 high and low groups. (F-G) The expressions of VDR and CCL20 detected by western blot. (H) Measurement of CCL20 concentration in the supernatants of VDR overexpression and VDR knockdown tumor cells by ELISA. (I) Prediction of VDR and CCL20 binding sites. (J) ChIP assay confirmed that VDR could bind to the CCL20 promoter in Panc1. (K) Schematic representation of the CCL20 promoter and its mutant site luciferin-reporter gene. (L) Luciferase assay demonstrated that VDR promoted CCL20 transcription. Values in the graphs are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X