Fig. 4

ImpL2 mediates suppression of insulin signaling. A: Lamellocyte number in animals with muscle specific knockdown or ImpL2^dsRNA compared to eGFP^dsRNA controls and and Muscle > ImpL2^dsRNA InR^DN animals 22 hpi (N = 3, n = 45–80), each dot represents a single larva. B: Percentage of viable Drosophila and wasp larvae in Muscle > eGFP^dsRNA, Muscle > ImpL2^dsRNA and Muscle > ImpL2^dsRNA InR^DN animals after wasp infection (N = 3, n = 51–55), there is no significant difference between number of surviving Drosophila larvae in Muscle > eGFP^dsRNA and Muscle > ImpL2^dsRNA, InR^DN; the number of melanized wasps in Muscle > ImpL2^dsRNA is significantly fewer (p = ≤ 0.0001) than the other genotypes. C: Expression of ImpL2 RA in control and upd animals with and without infection (n = 5), bars represent the mean values, each dot represents a biological replicate. D: Expression levels of ImpL2 RB with and without infection in control and upd animals (n = 5), bars represent the mean values, each dot represents a biological replicate. E: Number of lamellocytes in upd3 animals with (upd3Δ, Muscle > ImpL2) and without (upd3Δ, W¹¹¹⁸ > ImpL2) the overexpression of ImpL2 in muscle tissue (N = 3, n = 53–58), each dot represents a single larva. F: Resistance to wasp infection of upd3Δ, W¹¹¹⁸ > ImpL2 and Muscle > upd3Δ, Muscle > ImpL2 animals (N = 3, n = 53), p = 0.0283 for the difference between number of surviving Drosophila larvae. ns: no significant difference, *** p ≤ 0.001, **** ≤ 0.0001. N represents individual experiments, n represents biological replicates