Fig. 3

JAK/STAT signaling in muscles is mediated by insulin signaling. A: Number of lamellocytes in control and Stat92E^dsRNA animals (N = 3, n = 58–59), dots represent the number of lamellocytes of individual larvae. B: Comparison of resistance to wasp infection between control and Stat92E^dsRNA animals (N = 3, n = 43–55), the statistical difference in number of surviving Drosophila larvae (melanized wasp eggs + dead wasp larvae) between the two genotypes p = ≤ 0.0001. C: Relative expression of Thor in non-infected and infected control and upd mutant animals (n = 5), bars represent mean values with each dot showing a biological replicate. D: Lamellocyte number of upd3 mutant animals and animals of the same genetic background expressing InR^DN specifically in muscles as well as W¹¹¹⁸ > InR^DN and Muscle > InR^DN animals (N = 3, n = 97–133), dots represent the number of lamellocytes of individual larvae. E: Comparison of wasp resistance between upd3 animals with and without the expression of InR^DN in muscles and InR^DN expressed in control animals, statistical difference in number of surviving Drosophila larvae p = ≤ 0.0001; statistical difference of melanized wasp eggs between W¹¹¹⁸ > InR^DN and Muscle > InR^DNp = ≤ 0.01 (N = 3, n = 46–49). F: Relative expression of Thor in non-infected and infected control animals and upd mutants expressing InR^DN in muscles (n = 5), bars represent mean values with each dot showing a biological replicate. ns: no significant difference, * p ≤ 0.05, **** ≤ 0.0001. N represents individual experiments, n represents biological replicates