Fig. 6
Detection of PICALM in chorionic villi of placentas Ho-71 (normotensive) and Ho-73 (preeclamptic). A Top IGEM micrograph: partial profile of villus capillary from Ho-71. EC: endothelial cells. E: RBC, stroma. Bottom images: enlargement of two selected areas of endothelium show PICALM localization (arrows) at EC junctions (Ju), along EC plasma membrane, in cytoplasmic projections into lumen (L) and adjacent stroma (S). B Top micrograph: the nuclei of endothelium of a villus capillary from Ho-71, and the adjacent pericyte (P) are patent. Bottom image: enlargement of nucleus, shows PICALM in nucleoplasm near chromatin, on EC cytoplasmic membrane (white arrows) and in luminal space (black arrow). C Top micrograph: HC in stroma of Ho-73. E: RBC in capillary. At bottom: enlargement shows PICALM in HC nucleus (arrows). D Top micrograph: HC cell in the stroma of placenta Ho-71. Bottom image: enlargement shows PICALM in nucleoplasm in association with chromatin. E PICALM is shown (arrows) at the junction (Ju), and plasma membrane of EC and in adjacent stroma (S) of fetal capillary from Ho-73. F PICALM is shown (arrows) in cytoplasm and plasma membrane of EC of capillary from Ho-71, and in basal lamina (BL), stroma (S) and capillary lumen. The gold particle diameter is 10 nm