Fig. 5

Detection of MDMX by IGEM in chorionic villi of placentas Ho-71 (normotensive) and Ho-73 (preeclamptic). A Left micrograph: Nucleus (N) of endothelial cell (EC) from Ho-73. Arrow heads point to small MDMX clusters, parallel to the inner aspect of the EC plasma membrane. Area outlined, top left: EC junction, P: pericyte process, E: RBC in capillary lumen, S: stroma. This area is enlarged on the top right graph and shows MDMX clusters along the junction (Ju), and endoplasmic reticulum (ER). Bottom right: enlargement of outlined area shows diffuse pattern of MDMX clusters throughout the nucleoplasm. B Top left micrograph shows an outline of apoptotic macrophage in the lumen of fetal capillary (cap) from Ho-71. P: pericyte, F: partial profile of fibroblast (F) and HC partially marked by a white outline. Enlargement on the top right image shows diffuse MDMX localization in the vacuolated cytoplasm of luminal macrophage. Image at bottom left is enlargement of area outlined in white and shows MDMX clustering in the nucleus (N) and cytosol of the HC. Clusters, averaging 50–100 nm in diameter, run along the nuclear membrane and appear to be associated with mitochondrial and endoplasmic reticulum membranes. Bottom right: enlarged area shows MDMX clusters of approximately 100 nm. The gold particle diameter is 6 nm