Fig. 4
Detection of VEGFR2 by IGEM in chorionic villi of placenta Ho-73 (preeclamptic). A Top left micrograph shows a villous capillary. P: pericyte, E: RBC, SC: stromal cells. Bottom image details area outlined on the top micrograph and shows endothelial cell (EC) cytoplasm, pericyte (P), tight junction (*), collagen fibers in villous stroma (VS), and capillary lumen (L). Image at right: Enlargement of area outlined on the bottom left shows VEGFR2 in mitochondria (arrows). B Micrograph on top left shows villous capillary, stroma (S), luminal RBC (E), and Hofbauer cell (HC). Enlargement of rectangle at bottom shows prominent nucleolus, mitochondria, and endoplasmic reticulum of HC. Micrographs on the right show enlargement of areas outlined on the bottom left micrograph that detail clusters of VEGFR2 (arrows) in the cytosol (C), along segments of endoplasmic reticulum (ER), and mitochondria (M). Enlargement of image at bottom right shows diffuse localization of VEGFR2 clusters throughout the nucleoplasm (arrows). C Top left micrograph: Villus stroma depicting a fibroblast (Fb), and a pericyte (P) associated with fetal capillary in partial profile, RBC (E), and EC cytoplasm. Bottom left is enlargement of area outlined on the top graph and shows fibroblast cytosol, mitochondria (M), and villous stroma (S). To the right, enlargement of two areas show VEGFR2 in cytoplasm and in partial profiles of mitochondrial matrix (white arrow heads). D A TEM micrograph after osmication provided a better outline of mitochondria in an EC, compared to (A, B and C) panels. E Fetal capillaries with luminal RBC (E) are shown in the top left image, IS: intervillous space, mE: maternal RBC. Bottom: enlargement of outlined area shows a fetal macrophage in greater detail, a syncytiotrophoblast (SCT) is also present. Right micrograph: enlargement of area in bottom left micrograph, points to VEGFR2 labelling in an incomplete profile of a mitochondrion. Dashed arrows outline the outer double membrane of the labeled and unlabeled mitochondria. The gold particle diameter is 6 nm