Fig. 1

Morphological and biochemical characteristics of primary olfactory ensheathing cells (OECs) and microglial cells. a Phase-contrast microscopy showing morphology of primary OECs after purification at 5 days in vitro. b, c and d Double immunostaining with p75 and S100 in purified OECs, respectively. c Primary microglial cells at 3 days in vitro. e, f and g Representative photomicrographs of immunofluorescence for Iba1 and CD11b in purified microglial cells. h Percentages of p75⁺/S100⁺ cells among purified cells after sub-culture from passages 1–4. i Percentages of IBa1⁺/CD11b⁺ cells among purified cells after sub-culture from passages 1–4. The error bars indicate the SD of triplicate values. All data are reported as the means ± SEM. Scale bars, 100 mm