Fig. 5
GOLGA7 regulates anterograde trafficking of NRAS away from the Golgi (A) Anterograde trafficking of NRAS away from the Golgi was assessed via photoactivation of paGFP-NRAS on the Golgi (marked by mCherry-NRAS) and monitoring fluorescence loss over time; a representative cell is shown. Bars indicate 46.2 µm. B Curves trend the loss of initial fluorescence post-photoactivation in the region of the Golgi in cells ± sgGOLGA7 as mean ± SD for cells examined. Values are the means ± SD from n = 10 per group. C Retrograde trafficking of NRAS to the Golgi was examined using GFP-NRASG12D expressed in HeLa cells with FRAP kinetics determined in the region of the Golgi; a representative cell is shown. Bars indicate 46.2 µm. D Recovery curves trend the mean ± SD of the calculated fraction of initial fluorescence over time in the cells examined. Values are the means ± SD from n = 10 per group