Fig. 2From: GOLGA7 is essential for NRAS trafficking from the Golgi to the plasma membrane but not for its palmitoylationGOLGA7 knockout does not disturb the plasma localization of HRAS, KRAS4A and KRAS4B (A-C) Immunofluorescence showing the colocalization of the plasma membrane and GFP-HRASG12D (A), GFP-KRAS4AG12D (B) and GFP-KRAS4BG12D (C) in HeLa cells ± sgGOLGA7. The intensity profiles of GFP-HRASG12D (A), GFP-KRAS4AG12D (B) and GFP-KRAS4BG12D (C) / the plasma membrane were plotted in the right panel. Scale bars, 42.6 µm. D-F Statistical quantification of the colocalizations (Pearson’s coefficient) of GFP-HRASG12D/GFP-KRAS4AG12D/GFP-KRAS4BG12D and the plasma membrane. Values are the means ± SD from n = 15 per group, compared using two- tailed Student’s t test. GFP-HRASG12D GOLGA7-sg1, ns p = 0.9519; GFP-HRASG12D GOLGA7-sg2, ns p = 0.3184; GFP-KRAS4AG12D GOLGA7-sg1, ns p = 0.3668; GFP-KRAS4AG12D GOLGA7-sg2, ns p = 0.1120; GFP—KRAS4BG12D GOLGA7-sg1, ns p = 0.6088; GFP—KRAS4BG12D GOLGA7-sg2, ns p = 0.4221Back to article page