Fig. 1
GOLGA7 is required for the PM localization of NRAS (A) GFP-NRASG12D (green) was visualized by confocal microscopy in HeLa cells ± sgGOLGA7, the plasma membrane(red) is indicated with anti-Na+K+ATPase, the nuclei(blue) is indicated with staining of Hoechst 33,342. The intensity profiles of GFP-NRASG12D / the plasma membrane were plotted in the right panel. Scale bar, 42.6 µm. B Statistical quantification of the colocalizations (Pearson’s coefficient) of GFP-NRASG12D/ the plasma membrane. Values are the means ± SD from n = 15 per group, compared using two- tailed Student’s t test. GOLGA7-sg1, ****p < 0.0001; GOLGA7-sg2, ****p < 0. 0001. C Whole cell lysates (WCL) of HeLa cells ± sgGOLGA7 were subjected to fractionation for the detection of the subcellular localization of GFP-NRASG12D by Western blot analysis. GFP-NRASG12D were validated using anti-GFP. Subcellular fractions were validated using anti-Na+K+ATPase (PM) and anti-GAPDH (WCL). D Ectopic expression of HA-GOLGA7 in sgGOLGA7-targeted HeLa cell rescues proper NRAS trafficking. E Similar experiment of (A) in NRASG12D-transformed Ba/F3 cells