Fig. 3

CTGF plays an important role in CAFs to promote GC cell migration and invasion. A. A schematic diagram of a transwell migration/invasion assay in a co-culture system. B and C. The migration of SNU668 cells co-cultured with siRNA-transfected CAF104 (B) and CAF47 (C) was analyzed by transwell migration assay. The knockdown efficiency of CTGF was validated by qPCR and western blotting (Supplementary Fig. 4A–D) D. The invasion of SNU668 cells co-cultured with siRNA-transfected CAF104 was analyzed by transwell invasion assay. E. The migration of SNU668 cells incubated with different concentrations of CTGF was analyzed by transwell migration assay. Representative images of migrated or invaded cells on the membrane (magnification, 200x) are shown. Error bars indicate the standard deviation of representative triplicates from at least three experiments, which showed similar results. NT : non-transfected. NS: not significant, *: p < 0.05, **: p < 0.01, ***: p < 0.001