Table 1 List of qPCR primers for Osterix, OCN, OPN, and ALP
Gene | Primer Sequence |
|---|---|
Osterix | |
Forward | 5′-TCCCTGGATATGACTCATCCCT-3′ |
Reverse | 5′-CCAAGGAGTAGGTGTGTTGCC-3′ |
OCN | |
Forward | 5′-GGCGCTACCTGTATCAATGG-3′ |
Reverse | 5′-GTGGTCAGCCAACTCGTCA-3′ |
OPN | |
Forward | 5′-GGAGTTGAATGGTGCATACAAGG-3′ |
Reverse | 5′-CCACGGCTGTCCCAATCAG-3′ |
ALP | |
Forward | 5'-CCAACTCTTTTGTGCCAGAGA-3' |
Reverse | 5'-GGCTACATTGGTGTTGAGCTTTT-3' |
GAPDH | |
Forward | 5'-CCGCATCTTCTTTTGCGTCG-3' |
Reverse | 5'-GGACTCCACGACGTACTCAG-3' |