Fig. 6

The effect of miR-181b on osteoblastic differentiation and cell calcification of ACP. After inhibition of miR-181b, ACP cells were treated with Bmp2 (200 ng/ml) for 10 days. A The level of HDAC3 localized to the nucleus was detected by Western blotting, and histone served as the loading control. The nuclear localization of HDAC3 was detected by B immunofluorescence and a dual-luciferase reporter system (C). D Western blotting was used to measure Runx2 protein levels. E–H The mRNA levels of Osterix, OCN, OPN, and ALP were measured by PCR. I The calcification of ACP cells was detected by Alizarin red staining. *P < 0.05