Fig. 5

miR-181b targets CBX4 to suppress its expression. A PCR was performed to detect the expression levels of miRNAs which were predicted to target CBX4 by filtering the output of target prediction algorithms (TargetScan) in ACP cells with 200 ng/ml Bmp2 treatment for 10 days*vs control, P < 0.05, and ns = not significant. B The expression levels of the CBX4 protein were measured in ACP cells transfected with different miRNAs. C ACP cells with miRNAs knocked down by miRNA inhibitors (inh-181b, inh-200b, and inh-410) were treated with Bmp2 (200 ng/ml) for 10 days, and CBX4 protein levels were measured by Western blotting. D Sequences present in the 3’UTR of CBX4 targeted by miR-181b and its target region are highlighted. E miR-181b targeted to the segment of the 3’UTR of CBX4 decreased luciferase reporter gene activity in HeLa cells. *P < 0.05, and ns = not significant