Fig. 2

The expression of HDAC3 is increased in calcified ACP tissue. A ACP cells were treated with Bmp2 (0, 50, 100 and 200 ng/ml) for 10 days and then stained with 2% Alizarin red. B Western blotting was used to detect protein levels of HAT, HDAC1, HDAC2, HDAC3, and HDAC8 in ACP cells treated with Bmp2 (200 ng/ml) for 10 days. C, D HDAC3 protein levels were analyzed in noncalcified (n = 4) and calcified (n = 8) ACP tissue by Western blotting (left) and immunohistochemistry (right). In the IHC experiments, HDCA3 mainly showed strong cytoplasmic staining in calcified ACP samples. In contrast, in noncalcified ACP samples, the main area of HDAC3 expression was the nucleus. *P < 0.05