Fig. 6

The trans-interaction of Axl or Tyro3, but not receptor ligation is not involved in platelet activation. After incubation with 20 μg mL^− 1 anti-Gas6 antibody or isotype control IgG for 5 min, human platelets at a density of 2 × 10⁸ mL^− 1 were stimulated with 10 ng mL^− 1 poly(PHG) (a), 30 ng mL^− 1 convulxin (b), 0.1 μg mL^− 1 CRP (c), or 0.015 U mL^− 1 thrombin (d) to induce aggregation. Mean ± SEM, NS = not significant. n = 3, Student’s t-test. After human platelets were preincubated with 5 μg mL^− 1 control IgG, anti-Tyro3 Ab, anti-Axl Ab, and anti-Mertk Ab, respectively for 5 min, they were stimulated with 30 ng mL^− 1 convulxin to induce aggregation(e). After preincubation with 150 nM recombinant extracellular domain of Axl, Tyro3, and Mertk for 5 min, human platelets were stimulated with 30 ng mL^− 1 convulxin (f). Mean ± SEM, n = 3, NS = not significant, *P < 0.05, *** P < 0.001. One-way ANOVA and Tukey’s multiple comparison test