Fig. 10
From: The design of high affinity human PD-1 mutants by using molecular dynamics simulations (MD)
The hPD-L1 binding ability of hPD-1 mutants. The binding of hPD-1 mutants with hPD-L1-Fc were measured by FACS. a, c Representative flow cytometry analyses of hPD-L1 binding to the HEK-293 T cells expressing WT hPD-1 or the mutants. b, d The binding affinity between hPD-1 mutants and hPD-L1 at different protein concentrations. Each point represents the mean ± S.E. of four independent measurements. e1,e2 Relative PD-L1 binding potency (RP) values of the hPD-1 mutants. (mean ± S.E., n = 4). *, p < 0.05; **, p < 0.01 versus PD-1 (dashed line). RP is the ratio of the averaged MFI value of hPD-1 mutant to WT hPD-1 at 100 μM. The averaged MFI value was calculated from four independent measurements