Fig. 2From: Translationally controlled tumour protein TCTP is induced early in human colorectal tumours and contributes to the resistance of HCT116 colon cancer cells to 5-FU and oxaliplatinTreatment of HCT116 colon cancer cells with 5-FU and oxaliplatin results in increased TCTP protein levels. Cells were seeded in 12-well plates, grown to about 70% confluency and treated with either 5-FU or oxaliplatin at the indicated concentrations for 24 or 48 h. TCTP levels were assessed by western blotting. The bottom panels show representative western blots for TCTP and α-tubulin as a loading control. TCTP signals were quantified and normalised for the corresponding α-tubulin signals. In the graphs, normalised TCTP levels are plotted against the drug concentration for both the 24 h (filled symbols) and the 48 h treatment time (open symbols). Dots represent the average of three experiments ± SDBack to article page