Fig. 2
From: Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3
FAK inhibition causes mitochondrial dysfunction. a Pharmacological inhibition of the integrin signaling molecule, FAK, with FAK14 treatment of bEnd5 cells for 4 h produced a robust and dose-dependent suppression of mitochondrial bioenergetic function as shown in a representative OCR trace. Cells were grown for 44 h without substrate before treatment. b FAK14 causes reductions in basal respiration, ATP production, and maximum reserve and respiratory capacity. The cell number (c) and protein content (d) were not different across conditions. Cell counts were independently verified. e SiRNA-mediated knockdown of FAK (siFAK) decreased ATP production, reserve capacity and respiratory capacity relative to a non-targeting siRNA control (siControl). Data are mean ± SEM from 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant versus control vehicle group