Fig. 9
From: HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures
Knockdown of IQGAP1 rescue the defects of spreading and migration of Hectd1 R/R cells. a After 36 h of transfection, cells were starved overnight and plated on FN-coated cell culture dishes and immediately sent to time-lapse recording for 2 h (1 min/picture). Spreading pictures at different time points were shown. Note for cells with leading protrusion (yellow arrows). b Quantification of duration of cell spreading on 30 min is shown. AU, arbitrary unit. *, paired t test, P < 0.05. c 24 h after siRNA transfection, wound healing assays were performed. Migration images were acquired by time-lapse microscopy for 24 h. The images were analyzed quantitatively by Image J software (paired t test, P < 0.05)