Fig. 7
From: HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures
Overexpression of IQGAP1 affects focal adhesions formation. a Wild-type cells were transfected with GFP-IQGAP1 for 24 h. After starved overnight, cells were seeded on FN coated plate for 60 min and were subsequently fixed and stained with anti-paxillin and -zyxin, respectively. Non-transfected cells were used as control (arrow head). Note impaired formation of focal adhesions in GFP-IQGAP1 transfected cells (white arrows) compared with the control cells. b Quantification of average adhesions/cell is shown. Bar, 20 μm. *P < 0.05 (Mann-Whitney U test)