Fig. 4
From: HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures
Loss of HECTD1 leads to mislocalization of α-actinin and paxillin/zyxin. Equal amounts of wild-type and HECTD1 MEF cells were seeded on culture dishes for 24 h, followed by starvation overnight. The cells plated on FN for 60 min were co-stained with anti-α-actinin and anti-paxillin or anti-zyxin, respectively. The dotted frame was zoomed out at the right panel, and the colocalization of two proteins across the dashed line was shown in the fluorescence intensity profiles. Bar, 20 μm