Fig. 3
From: HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures
Loss of HECTD1 leads to mislocalization of paxillin and zyxin. a Expression of paxillin or zyxin was determined by Western Blots. b Total focal adhesion area was determined by Image J. c Wild-type and Hectd1 R/R MEF cells were seeded on coverslips pre-coated with 1 μg/ml FN for 2 h, followed by anti-paxillin or anti-zyxin staining. Bar, 50 μm. d Amount of paxillin and zyxin and the individual focal adhesion were analyzed by Image J software. All the experiments were repeated at least 3 times and over 50 cells were analyzed in each group. Mann-Whitney U test were conducted. e In the same condition, cells were stained with anti-paxillin (phosphor Y118) and rhodamine phalloidin