Fig. 2
From: HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures
Mutant HECTD1 impairs directional cell migration. a Wound healing assays were performed on FN for 36 h in the medium containing 1 μM aphidicolin. b Velocity of cell migration was quantified by Image J software (paired t test, *P < 0.05). c Loss of HECTD1 impairs straightness of directional cell migration. Up and down plots of cell migration trajectories, velocity and straightness were measured by Manual tracking and chemotaxis tool (Image J). AU, arbitrary unit (paired t test, *P < 0.05). d Wound healing experiments were manipulated in 24 well plates with coverslips. The orientations of MTOC were indicated with white arrows. The percentage of MTOC orientated towards the wound was determined at 10 h post wounding (paired t test, *P < 0.05). Cells were fixed with 4% paraformaldehyde then co-stained with acetylated alpha tubulin and Giantin antibodies. Bar, 50 μm