Fig. 1
From: HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures
Fibronectin is a critical extracelluar matrix in HECTD1 regulating cell adhesion and the mutant HECTD1 accelerates cell spreading. a Wound healing assay. Equal amount of wild-type and Hectd1 R/R MEF or Hela cells were seeded on 24 well plates coated with various ECMs for 24 h with 0.5% FBS, followed by starvation overnight with 1 μg/ml aphidicolin (see Methods). Wounds were created by 200 μl pipette tips and placed into a complete medium containing 10% FBS and aphidicolin. Migration images were acquired by time-lapse microscopy for 24 h. FN indicates fibronectin, CL1 stands for collagen type I, CL4 for collagen IV, MT for matrigel, LM for laminin and GL for gelatin. Experiments for each ECM were conducted for at least three times (paired t test, *P < 0.05). b Wild-type and Hectd1 R/R cells were starved overnight, then plated on FN coated plates and immediately sent to time-lapse microscopy for recording 2 h (1 min / picture). Spreading on different time points were shown. c Duration of cell spreading was quantified by Image J software (paired t test, *P < 0.05)