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Table 1 Influence of US28 coexpression on efficacy and potency of CXCL12-induced cAMP concentrations

From: Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28

Cotransfection

Emax (mean ± SEM)

pEC50 (mean ± SEM)

n

CXCR4 + mock (control)

100 ± 4

9.85 ± 0.09

8

CXCR4 + US28

32 ± 11 (***)

9.54 ± 0.91 (n.s.)

4

CXCR4 + US28Δ300

35 ± 18 (***)

8.96 ± 1.10 (n.s.)

3

CXCR4 + US28DQY

73 ± 7 (n.s.)

10.02 ± 0.22 (n.s.)

5

CXCR4 + US28Δ300/DQY

76 ± 8 (n.s.)

10.34 ± 0.26 (n.s.)

4

  1. Data shown correspond to Fig. 1a. Efficacy (Emax) is calculated as the absolute value of the maximal CXCL12-induced effect normalized on CXCR4-only expressing cells (100%). Potency is displayed as pEC50. Data were derived from three to eight independent experiments each performed in triplicate wells and are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc test comparing US28 coexpressing systems with CXCR4-only expressing cells (control). ***P < 0.001, n.s. not significant
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Cell Communication and Signaling

ISSN: 1478-811X