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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Epigenetic regulation of transcription factor promoter regions by low-dose genistein through mitogen-activated protein kinase and mitogen-and-stress activated kinase 1 nongenomic signaling

Fig. 3

Colocalization of phosphorylated (p) MSK1 and H3S10ph in ht-UtLM cells treated with genistein (1 μg/ml). a The immunofluorescence staining was performed to detect Phospho-MSK1 (green) and H3S10ph (red) colocalization in ht-UtLM cells in the presence or absence of the PD inhibitor following genistein exposure at 0 min (control) and 10 min. Inset: Negative control with normal rabbit serum. b The intensity (%) and positive staining area (%) of phospho-MSK1 and H3S10ph induced by genistein, and the Pearson’s coefficiency of colocalization (r) of pMSK1 and H3S10ph colocalized signals. Data present the mean+/-SEM of percentage (%) of positive staining area and intensity from scanned images; *p < 0.05 treated 10 min versus non-treated 0 min. Blue = DAPI nuclear stain. Bar = Magnification in μm

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Cell Communication and Signaling

ISSN: 1478-811X