Fig. 2

Inhibition of platelet PKG activation in the presence of RBCs. a FACS analysis of VASP phosphorylation at S²³⁹ measured in the whole blood, PRP, washed platelets, and WP with washed RBCs (10⁹/ml). b VASP phosphorylation at S²³⁹ measured in the whole blood in the presence of L-arginine or NOS inhibitor L-NAME (both 200 μM, 10 min preincubation). NO donor (DEA-NO, 5 μM, 2 min) was used as positive control. Samples were prepared as described under “Materials and Methods”. Data are presented as mean ± SEM, n = 4; ⁺ p < 0.05, compared to control