Fig. 5
From: Differential Lyn-dependence of the SHIP1-deficient mast cell phenotype
Lyn/Ca2+/calcineurin-dependent signaling controls Ag-induced transcription of various inflammatory genes. BMMCs were preloaded and starved overnight with 0.15 μg/ml IgE. a Wt, Lyn-/-, SHIP1-/-, and dko BMMCs were left untreated (con) or stimulated with 20 ng/ml of Ag for 90 min. The amounts of Tnfsf9 mRNA, Tnip3 mRNA, Il1a mRNA, Il1b mRNA, and Stx11 mRNA were measured by RT-qPCR. A comparison of analyses of different independent cell cultures is depicted in Additional file 8: Figure S8. b SHIP1-/- BMMCs, pretreated with vehicle or 100 nM CsA for 30 min, were left unstimulated (con) or stimulated with 200 ng/ml Ag for 90 min. The amounts of Tnfsf9 mRNA, Tnip3 mRNA, Il1a mRNA, Il1b mRNA, and Stx11 mRNA were measured by RT-qPCR. Comparable results were obtained with cells from different BMMC cultures (n = 3 (a); n > 3 (b)