Fig. 1

a MDA-MB-231, Sk-Br-3, T47D and MCF-7 tumor cells were treated with cisplatin (0.1-50 μg/ml) diluted in standard culture medium. The concentration of 1 μg/ml cisplatin (IC80 for almost all used cell lines) was set as a concentration used for pretreatment of MSC. b MSC were pretreated with 1 μg/ml cisplatin or 10-fold higher dose (10 μg/ml). Measurement of Caspase-3/7 activity has shown that MSC are resistant to 1 μg/ml cisplatin by corresponding to the induction of apoptosis in evaluated cells. The treatment with 1 μg/ml cisplatin did not trigger apoptosis in pretreated MSC within 48 h. MSC underwent apoptosis after more than 15 h exposure to 10 μg/ml cisplatin. c Using the IncuCyte Zoom™ Kinetic Imaging System we have shown that the morphology of cells treated with 1 μg/ml cisplatin remained unchanged to control and the pretreatment did not induce activation of fluorescence in cells because of missing caspase-3/7, in comparison to MSC treated with 10 μg/ml cisplatin