Figure 6
From: The structure function of the death domain of human IRAK-M
Effect of human IRAK-M and death domain mutants in lung epithelial cells. WT IRAK-M and DD-mutants were stably expressed in the lung epithelial cell line H292 by lentiviral transduction and FACS-sorting of EGFP positive cells which is bicistronically expressed. (A) IRAK-M expression evaluated by western blotting. (B) The effect of stable IRAK-M expression and IRAK-M mutants was determined on IL-1β and Flagellin (TLR5) mediated IL-8 expression in the supernatant after 6 hour stimulation. Shaded bars depict results of IRAK-M molecules with combinations of mutated residues/stretches within the same area. N=8, mean±SEM. * indicates difference with WT IRAK-M P<0.05. (C) Effect of stable expression of WT and DD domain mutants on IL-8 mRNA levels induced by Flagellin after 3 hours. N=3, mean±SEM.