Figure 1

MiR-188 inhibits cell proliferation and tumor colony formation. (A) Overexpression of miR-188 inhibits cell growth in CNE cells. CNE cells were transfected with miR-188 or miR-NC for 72 h, the representative phase contrast images were shown. Scar bar: 250 μm. (B) Overexpression of miR-188 inhibits cell proliferation. Equal amounts of CNE cells were transfected with miR-188 or miR-NC, cell proliferation was monitored at indicated time points. Data presented are means ± SD from three independent experiments (Student t test, *p < 0.05, **p < 0.01). (C) Clonogenic assay of CNE cells transfected with miR-NC or miR-188. Cells were cultured for 10 days and stained with Crystal Violet. (D) The number of colonies were quantified and calculated as means ± SD of three independent experiments (Student t test, ***p < 0.001). (E) Clonogenic assay of CNE cells stably expressing miR-NC or miR-188. (F) Stable transfection of miR-188 inhibits cell proliferation. Equal amounts of miR-NC or miR-188 stable cell lines were cultured for 4 days, cell proliferation was monitored at indicated time points. Data shown are means ± SD from three independent experiments (Student t test, *p < 0.05, **p < 0.01, ***p < 0.001). (G) The functional association of miR-188 target genes. DAVID Functional Annotation tool was used for analysis of potential targets predicted from Targetscan and Findtar. A series of cell cycle related genes were potential targets of miR-188.