Figure 6

Lipid raft coalescence is cholesterol-dependent. Lipid rafts were isolated from YH16.33 cells co-cultured with A20 cells in the absence (A & B) and presence (C & D) of chicken ovalbumin after the treatment of co-cultured cells with MβCD for 15 minutes. Lipid rafts from fraction 5 of the density gradient were captured with anti-Thy-1 on formvar coated nickel grids and detected with biotinylated anti-Ly-6A.2 followed by anti-biotin colloidal gold conjugate. A representative micrograph (3 independent experiments) of lipid rafts (shown by arrows) from MβCD treated YH16.33 cells co-cultured with A20 cells in the absence (A) and presence (C) of antigen is shown. The average Feret's diameter of lipid rafts generated from YH16.33 and A20 co-cultures in the absence (C) and presence of antigen (D) is shown. Each micrograph was 40,000 × magnification.